National Standard Methods in PDF Format
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COMMENT ON NATIONAL STANDARD METHODS
KEY:
I = Issued
UR / I = Issued and Under Review
UR = Under Review
D = Draft
WD = Withdrawn
C = Contact Standards Unit
- Bacteriology
- Bacteriology Identification
- Food
- Guidance Notes
- Milk and Dairy
- Test Procedures
- Virology
- Water
National Standard Methods – Bacteriology SOPs
Some National Standard Methods are available in Italian
Ref No | Title | Status |
Issue No |
Issue Date |
Investigation of ear swabs and associated specimens |
I |
8.1 |
Mar 08 |
|
Investigation of eye swabs and canalicular pus |
I |
5 |
Mar 08 |
|
Investigation of mouth swabs |
I |
6 |
Feb 08 |
|
Investigation of nose swabs |
I |
6 |
Feb 08 |
|
Investigation of specimens for Bordetella species |
I |
6.1 |
May 05 |
|
Investigation of throat swabs |
UR |
N/A |
N/A |
|
Processing of faeces for Clostridium difficile |
I |
1.2 |
Mar 08 |
|
Investigation of skin, superficial, and non-surgical wound swabs |
I |
4.1 |
Aug 06 |
|
Investigation of abscesses and post-operative wound and deep-seated wound infections |
UR |
N/A |
N/A |
|
Investigation of bile |
I |
5 |
Apr 08 |
|
Investigation of tissues and biopsies |
UR |
N/A |
N/A |
|
Investigation of sinus aspirate |
UR |
N/A |
N/A |
|
Investigation of intravascular cannulae and associated specimens |
I |
5 |
May 08 |
|
Investigation of cerebrospinal fluid shunts |
UR |
N/A |
N/A |
|
Investigation of gastric aspirates and infection screen swabs from neonates |
UR |
N/A |
N/A |
|
Investigation of continuous ambulatory peritoneal dialysis fluid |
UR |
N/A |
N/A |
|
Investigation of fluids from normally sterile sites |
I |
4.1 |
May 05 |
|
Investigation of cerebrospinal fluid |
I |
5 |
Apr 08 |
|
Investigation of genital tract and associated specimens |
I |
4.1 |
May 05 |
|
Investigation of specimens for screening for MRSA |
I |
5 |
Jul 07 |
|
Investigation of faeces specimens for bacterial pathogens |
I |
6 |
Sep 07 |
|
Investigation of specimens other than blood for parasites |
UR |
N/A |
N/A |
|
Investigation of Specimens For Ectoparasites |
I |
1 |
Jun 06 |
|
Investigation of blood cultures (for organisms other than Mycobacterium species) |
I |
5 |
Aug 05 |
|
Investigation of Bone Marrow |
I |
1 |
Nov 07 |
|
Investigation of dermatological specimens for superficial mycoses |
I |
1 |
Mar 07 |
|
Investigation of specimens for Mycobacterium species |
I |
5 |
Jul 06 |
|
Investigation of Urine |
I |
6 |
May 08 |
|
Investigation of bone and soft tissue associated with Osteomyelitis |
D |
N/A |
N/A |
|
Investigation of Prosthetic Joint Infection samples |
D |
N/A |
N/A |
|
Susceptibility Testing |
I |
2 |
Oct 06 |
|
Investigation of specimens for Legionella species |
UR |
N/A |
N/A |
|
Screening for Meningococci |
I |
1.1 |
May 05 |
|
Investigation of intraocular fluids and corneal scrapings |
I |
5 |
Apr 08 |
|
Investigation of gastric biopsies for Helicobacter pylori |
UR |
N/A |
N/A |
|
Investigation of Bronchoalveolar Lavage, Sputum and Associated Specimens |
I |
2 |
May 08 |
|
Processing Swabs For Group B Streptococcal Carriage |
I |
2.1 |
Jun 06 |
Number of Issued Bacteriology SOPs: 25
National Standard Methods – Bacteriology Identification
Ref No | Title | Status |
Issue No |
Issue Date |
Introduction to the preliminary identification of medically important bacteria |
I |
1.4 |
Feb 08 |
|
Identification of Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis |
D |
N/A |
N/A |
|
Identification of Listeria species, non-toxigenic Corynebacterium species and other non-sporing gram-positive rods |
I |
2 |
Oct 07 |
|
Identification of Streptococcus species, Enterococcus species and morphologically similar organisms |
I |
2.1 |
Sep 07 |
|
Identification of Bordetella pertussis and Bordetella parapertussis from selective agar |
I |
2.1 |
Sep 07 |
|
Identification of Neisseria species |
UR |
N/A |
N/A |
|
Identification of Staphylococcus species, Micrococcus species and Rothia species |
I |
2.1 |
Sep 07 |
|
Identification of Clostridium species |
UR |
N/A |
N/A |
|
Identification of Bacillus species |
I |
2.1 |
Sep 07 |
|
Identification of aerobic actinomycetes |
D |
N/A |
N/A |
|
Identification of Moraxella species and morphologically similar organisms |
I |
2 |
Aug 07 |
|
Identification of Haemophilus species and the Hacek group of organisms |
UR |
N/A |
N/A |
|
Identification of Pasteurella species and morphologically similar bacteria |
I |
2.1 |
Sep 07 |
|
Identification of non-sporing, non-branching anaerobes |
D |
N/A |
N/A |
|
Identification of anaerobic actinomycetes |
D |
N/A |
N/A |
|
Identification of Enterobacteriaceae |
I |
2 |
Nov 07 |
|
Identification of glucose non-fermenting Gram-negative rods |
I |
2 |
Apr 07 |
|
Identification of Legionella species |
I |
2 |
Nov 07 |
|
Identification of Vibrio species |
I |
2 |
Nov 07 |
|
Identification of Shigella species |
I |
2 |
Nov 07 |
|
Identification of Yersinia species from faeces |
I |
2 |
Nov 07 |
|
Identification of Escherichia coli O157 |
I |
2 |
Nov 07 |
|
Identification of Campylobacter species |
I |
2 |
Nov 07 |
|
Identification of Salmonella species |
I |
2 |
Nov 07 |
|
Identification of anaerobic Gram-negative rods |
D |
N/A |
N/A |
|
Identification of Helicobacter species |
I |
2 |
Feb 08 |
Number of Issued Bacteriology ID SOPs: 18
National Standard Methods – Food
Ref No | Title | Status |
Issue No |
Issue Date |
Preparation of Samples and Dilutions |
I |
1.4 |
May 05 |
|
Aerobic Plate Count at 30°C: Surface Plate Method |
I |
1.4 |
May 05 |
|
Aerobic Plate Count at 30°C: Spiral Plate Method |
I |
1.4 |
May 05 |
|
Enumeration of Staphylococcus aureus |
I |
1.4 |
May 05 |
|
Detection of Salmonella species |
I |
3 |
Jun 07 |
|
Enumeration of Clostridium perfringens |
I |
2.4 |
May 05 |
|
Enumeration of Bacillus cereus and other Bacillus species |
I |
1.4 |
May 05 |
|
Enumeration of Escherichia coli in Raw Molluscs by the Multiple Tube Technique |
I |
4.2 |
May 05 |
|
Detection of Escherichia coli O157 by Automated Immunomagnetic Bead Separation |
I |
3 |
May 08 |
|
Detection and Enumeration of Enterobacteriaceae |
I |
1 |
Dec 05 |
|
Detection and Enumeration of Listeria monoctyogenes & other Listeria species |
I |
2 |
Dec 07 |
|
Direct Enumeration of Escherichia coli |
I |
1.4 |
May 05 |
|
Detection of Campylobacter species |
UR |
N/A |
N/A |
|
Enumeration of ß-glucuronidase positive Escherichia coli – Most Probable Number method |
I |
1 |
Dec 05 |
|
Enumeration of Enterobacteriaceae by the Colony Count Technique |
I |
1.4 |
May 05 |
Number of Issued Food SOPs: 14
National Standard Methods – Guidance Notes
Ref No | Title | Status |
Issue No |
Issue Date |
|
QSOP 2 | Local document control of documents issued by Technical Services. The Health Protection Agency |
UR / I
|
4.2
|
Sep 05
|
|
QSOP 4 | Uncertainty of Measurement in Testing |
I
|
5
|
Aug 05
|
|
QSOP 5 | Handling and transport of samples (for the purposes of UKAS accreditation) |
UR / I
|
3.2
|
Sep 05
|
|
QSOP 10 | Traceability and use of thermometers in UKAS accredited laboratories |
I
|
4
|
Aug 07
|
|
QSOP 12 | Avoiding cross-contamination risks in the food laboratory |
UR / I
|
4.2
|
Sep 05
|
|
QSOP 13 | Auditing in Health Protection Agency laboratories |
UR
|
N/A
|
N/A
|
|
QSOP 17 | Handling of formal food samples in the laboratory |
I
|
4.1
|
Aug 05
|
|
QSOP 18 | Recommended minimum internal quality control in food microbiology testing laboratories |
UR / I
|
4.2
|
Sep 05
|
|
QSOP 19 | Specification for food and dairy products request forms |
I
|
4.1
|
Aug 05
|
|
QSOP 20 | Specification and standard comments for dairy products report forms |
I
|
4.1
|
Aug 05
|
|
QSOP 21 | Specification and standard comments for food report forms |
UR / I
|
3.2
|
Sep 05
|
|
QSOP 22 | Minimum performance testing requirements for food and dairy standard methods |
UR / I
|
3.2
|
Sep 05
|
|
QSOP 23 | Commercial and in-house diagnostic tests: Evaluations and Validations |
I
|
4
|
Jan 07
|
|
QSOP 27 | Quality assurance in the diagnostic virology and serology laboratory |
I
|
5
|
Feb 07
|
|
QSOP 29 | Protocol for involvement of HPA Laboratories and staff in the investigation of food, dairy products and waterborne disease |
UR / I
|
3.2
|
Sep 05
|
|
QSOP 30 | Laboratory diagnosis of legionella infections in the HPA |
WD
|
N/A
|
N/A
|
|
QSOP 31 | Surveillance of polio in the UK |
I
|
3.3
|
Nov 05
|
|
QSOP 33 | European Directive on in vitro diagnostic medical devices (98/79/EC) |
I
|
4
|
Jul 07
|
|
QSOP 36 | Corrective and preventive action |
I
|
4
|
Jan 07
|
|
QSOP 37 | Procedure for dealing with presumptive pathogens isolated from ready-to-eat foods |
I
|
4.1
|
Aug 05
|
|
QSOP 38 | Good laboratory practice when performing molecular amplification assays |
I
|
3
|
Aug 06
|
|
QSOP 39 | Food and water law sources for food, water and environmental laboratories |
UR / I
|
4.1
|
Sep 05
|
|
QSOP 42 | Microbiological examination of CSFs, that may contain agents of spongiform encephalopathies |
UR / I
|
2.2
|
Sep 05
|
|
QSOP 44 | Microbiological investigation of patients with acute lymphadenopathy and fever |
UR
|
N/A
|
N/A
|
|
QSOP 45 | Installation, operation, maintenance and performance testing of the Dynal Bead Retriever |
I
|
2.1
|
Apr 08
|
|
QSOP 46 | Aminoglycoside and glycopeptide assays in clinical microbiology laboratories |
I
|
1
|
Jan 07
|
|
QSOP 47 | Chlamydial Zoonotic Infections |
D
|
N/A
|
N/A
|
|
QSOP 48 | Investigation of Viral Encephalitis and Meningitis |
UR
|
N/A
|
N/A
|
|
QSOP 49 | Safe use of plastic bags for incubation of food samples |
I
|
1.1
|
Oct 07
|
|
QSOP 50 | Requirements of Food Examiners, Experts Witnesses and Witnessess of Fact Acting For/On Behalf of the Health Protection Agency |
I
|
1.2
|
Aug 05
|
|
QSOP 51 | Laboratory detection and reporting of bacteria with extended spectrum ß-Lactamases |
I
|
2.2
|
May 08
|
|
QSOP 52 | Inoculation of culture media (formerly BSOP 54) |
I
|
1
|
Feb 08
|
|
QSOP 53 | Recommendations for the screening of specimens for Corynebacterium species |
UR
|
1
|
Aug 05
|
|
QSOP 55 | Investigation of Vesicular Rashes |
I
|
1
|
Nov 05
|
|
QSOP 56 | Investigation of Red Rash |
I
|
1
|
Jan 07
|
|
QSOP 57 | The Microbiological Examination of Water Samples |
UR / I
|
2
|
Dec 05
|
|
QSOP 58 | Presumptive diagnosis of Enterobacter sakazakii infection associated with powdered infant formula |
UR
|
N/A
|
N/A
|
|
QSOP 59 | Investigation of Toxoplasma Infection in pregnancy |
I
|
1
|
Nov 06
|
|
QSOP 60 | Respiratory viruses |
D
|
N/A
|
N/A
|
|
QSOP 63 | Assessment of point of care tests in Microbiology |
D
|
N/A
|
N/A
|
Number of Issued Guidance Notes SOPs: 31
National Standard Methods – Test Procedures
Ref No | Title | Status |
Issue No |
Issue Date |
Aesculin hydrolysis test |
I |
1.1 |
May 05 |
|
Agglutination test |
UR |
1.1 |
May 05 |
|
Bile solubility test |
UR |
1.1 |
May 05 |
|
Catalase test |
UR |
1.2 |
Jun 06 |
|
Coagulase test |
UR |
3 |
Oct 05 |
|
Deoxyribonuclease test |
UR |
1.1 |
May 05 |
|
Indole test |
UR |
1.1 |
May 05 |
|
Motility test |
UR |
1.1 |
May 05 |
|
Nagler test |
UR |
1.1 |
May 05 |
|
ONPG (ß-galactosidase) test |
UR |
1.1 |
May 05 |
|
Optochin test |
UR |
N/A |
N/A |
|
Oxidase test |
UR |
N/A |
N/A |
|
Oxidation and fermentation of glucose test |
UR |
N/A |
N/A |
|
Porphyrin synthesis (ala) test |
UR |
N/A |
N/A |
|
Potassium hydroxide test |
UR |
N/A |
N/A |
|
Changing the phase of Salmonella test |
UR |
N/A |
N/A |
|
Thermonuclease activity test |
UR |
N/A |
N/A |
|
Urease test |
UR |
N/A |
N/A |
|
X and V factor test |
UR |
N/A |
N/A |
|
Staining Procedures |
I |
1 |
Mar 07 |
Number of Issued Test Procedures SOPs: 2
National Standard Methods – Milk and Dairy
Ref No | Title | Status |
Issue No |
Issue Date |
Preparation of samples and decimal dilutions |
I |
2.4 |
May 05 |
|
Plate count test at 30°C |
I |
3.1 |
May 05 |
|
Plate count test at 21°C (pre-incubated) |
I |
3.1 |
May 05 |
|
Enumeration of coliforms – colony count at 30°C |
I |
2.4 |
May 05 |
|
Enumeration of coliforms and presumptive Escherichia coli by the most probable number (MPN) technique |
I |
2.4 |
May 05 |
|
Determination of peroxidase activity (Storch test) in milk |
I |
2.4 |
May 05 |
|
Determination of alkaline Phosphatase Activity in Pasteurised Milk and Cream – Fluorimetric Method |
I |
1.4 |
May 05 |
Number of Issued Milk and Dairy SOPs: 7
National Standard Methods – Virology
Ref No | Title | Status |
Issue No |
Issue Date |
Gastroenteritis: sporadic cases |
I |
5 |
Jul 07 |
|
Gastroenteritis: Outbreaks |
I |
6 |
Aug 07 |
|
Hepatitis B diagnostic serology in the immunocompetent (including Hepatitis B in pregnancy |
UR / I |
4.1 |
June 03 |
|
Investigation of Hepatitis C Infection |
UR |
N/A |
N/A |
|
Hepatitis, jaundice and abnormal LFTs |
I |
2.2 |
Nov 05 |
|
Vertical HCV transmission algorithm |
I |
1 |
Nov 07 |
|
Blood borne virus testing in dialysis patients |
I |
1 |
Feb 08 |
|
Anti-HIV Screening |
UR |
N/A |
N/A |
|
Preparation of coated grids for electron microscopy |
I |
5.1 |
Aug 06 |
|
Electron Microscopy: Flotation (direct) method |
I |
5 |
Feb 07 |
|
Electron Microscopy: Differential Centrifugation (double-spin method) |
I |
5 |
Feb 07 |
|
Electron microscopy: ammonium sulphate precipitation |
I |
5 |
Sep 07 |
|
Investigation of clinical specimens by electron microscopy using solid phase immune electron microscopy |
I |
5 |
Sep 07 |
|
Isolation of herpes simplex virus associated with herpes genitalis |
I |
4 |
Jul 07 |
|
Complement fixation tests |
I |
2 |
Jul 06 |
|
Nucleic Acid Extraction by the Boom Method |
UR |
N/A |
N/A |
|
Isolation of Viruses associated with infections of the eye: Keratoconjunctivitis |
I |
2 |
Feb 07 |
|
Immunofluorescence and isolation of viruses from respiratory samples |
I |
1 |
Jul 07 |
|
Investigation of human herpes viruses (excluding herpes genitalis) |
I |
2 |
Feb 06 |
|
Isolation of enteroviruses and parechoviruses |
I |
2 |
May 06 |
|
Real-Time Quadriplex PCR for the detection of Influenza |
C |
1 |
Nov 06 |
|
Epstein-Barr Virus serology (formerly VSOP 6.2) |
I |
3 |
Mar 08 |
|
Hepatitis A virus acute infection serology (formerly VSOP6.3) |
I |
2.4 |
Oct 07 |
|
Cytomegalovirus serology (formerly VSOP6.4) |
I |
2.4 |
Oct 07 |
|
Investigation of erythrovirus (parvovirus) B19 in pregnant women exposed to rash illness |
UR |
N/A |
N/A |
|
Management of confirmed erythrovirus (parvovirus) B19 infection in pregnancy |
UR |
N/A |
N/A |
|
Investigation of rubella in pregnant women of unknown rubella immunity status exposed to rash illness |
UR |
N/A |
N/A |
|
Pregnant patient in contact with rash illness |
I |
2.2 |
May 05 |
|
Investigation of pregnant patient with rash illness |
I |
2.2 |
May 05 |
|
Investigation of Cytomegalovirus infection by Roche Lightcycler PCR |
I |
2.1 |
Aug 07 |
|
Operation of the Roche MagNA Pure LC automated nucleic acid extraction robot |
I |
2 |
May 06 |
|
Chlamydia Infection – Testing by Nucleic Acid Amplification Test (NAATs) |
I |
2 |
Feb 08 |
|
Detection of SARS-CoV by reverse transcription PCR |
C |
1.2 |
May 05 |
|
Procedure for the care and propagation of cell cultures for virus isolation |
I |
1 |
May 06 |
|
RT-PCR (Block Based) for Influenza H5 viruses |
I |
3.3 |
Nov 06 |
|
Real-Time RT-PCR (Taq Man) for influenza A H5 viruses |
I |
4.1 |
May 06 |
|
RT-PCR for the detection of influenza A viruses |
I |
1.3 |
May 06 |
|
Investigation of viral encephalitis |
UR |
N/A |
N/A |
|
Serological Diagnosis of Syphilis |
I |
1 |
Nov 07 |
|
Haemadsorption of viruses |
I |
1 |
Aug 06 |
|
European Real-Time RT-PCR for Influenza A H5 viruses |
I |
1.1 |
May 06 |
Number of Issued Virology SOPs: 32
National Standard Methods – Water
Ref No | Title | Status |
Issue No |
Issue Date |
General technique for the detection and enumeration of bacteria by negative pressure membrane filtration |
I |
4.1 |
Apr 07 |
|
Enumeration of coliform bacteria and Escherichia coli by membrane filtration |
I |
4.1 |
Oct 07 |
|
Enumeration of enterococci by membrane filtration |
I |
3 |
Aug 06 |
|
Colony count by the pour plate method |
UR |
N/A |
N/A |
|
Enumeration of Clostridium perfringens by membrane filtration |
I |
3.1 |
May 05 |
|
Enumeration of Pseudomonas aeruginosa by membrane filtration |
I |
3 |
Dec 07 |
|
Detection of Salmonella species |
I |
3 |
Jul 06 |
|
Detection of Campylobacter species in water |
I |
3 |
Oct 07 |
|
Enumeration of Aeromonas species by membrane filtration |
I |
1.3 |
May 05 |
|
Enumeration of Staphylococcus aureus by membrane filtration |
I |
3.3 |
May 05 |
|
Detection and enumeration of Legionella species by filtration and centrifugation |
I |
1.2 |
Aug 06 |
|
Detection and enumeration of Legionella species by centrifugation |
I |
1.2 |
Aug 06 |
|
Detection and enumeration of Legionella species by positive pressure membrane filtration |
I |
1.2 |
Aug 06 |
|
Detection and enumeration of Legionella species in biofilms and sediments |
I |
1.2 |
Aug 06 |
|
Detection of Escherichia coli O157 by automated immunomagnetic separation |
I |
4 |
Mar 06 |
|
Isolation and identification of Acanthamoeba species |
I |
2.3 |
May 05 |
|
Enumeration of coliforms and Escherichia coli by Idexx (Colilert 18) Quanti-TrayTM |
I |
2.3 |
May 05 |
|
Enumeration of viable micro-organisms: Aerobic colony count by membrane filtration method |
I |
1 |
Dec 07 |
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